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Registro Completo |
Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
Data corrente: |
26/03/2004 |
Data da última atualização: |
05/05/2022 |
Autoria: |
MATSUMOTO, K.; VILARINHOS, A. D.; OKA, S. |
Afiliação: |
KAZUMITSU MATSUMOTO, Cenargen; ALBERTO DUARTE VILARINHOS, CNPMF. |
Título: |
Somatic hybridization by electrofusion of banana protoplasts. |
Ano de publicação: |
2002 |
Fonte/Imprenta: |
Euphytica, v.125, p.317-324, 2002. |
Idioma: |
Inglês |
Palavras-Chave: |
Huibrido Embryogenesis; Protoplasto fusão; Somatic hybrid. |
Thesagro: |
Banana; Embriogénese. |
Thesaurus Nal: |
Musa; protoplast fusion. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/177828/1/ID-240621.pdf
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Marc: |
LEADER 00596naa a2200217 a 4500 001 1176785 005 2022-05-05 008 2002 bl uuuu u00u1 u #d 100 1 $aMATSUMOTO, K. 245 $aSomatic hybridization by electrofusion of banana protoplasts.$h[electronic resource] 260 $c2002 650 $aMusa 650 $aprotoplast fusion 650 $aBanana 650 $aEmbriogénese 653 $aHuibrido Embryogenesis 653 $aProtoplasto fusão 653 $aSomatic hybrid 700 1 $aVILARINHOS, A. D. 700 1 $aOKA, S. 773 $tEuphytica$gv.125, p.317-324, 2002.
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Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Milho e Sorgo. Para informações adicionais entre em contato com cnpms.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Milho e Sorgo. |
Data corrente: |
09/09/2014 |
Data da última atualização: |
23/05/2017 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
GAZAFFI, R.; MARGARIDO, G. R. A.; PASTINA, M. M.; MOLLINARI, M.; GARCIA, A. A. F. |
Afiliação: |
MARIA MARTA PASTINA, CNPMS. |
Título: |
A model for quantitative trait loci mapping. |
Ano de publicação: |
2014 |
Fonte/Imprenta: |
Tree Genetics & Genomes, v. 10, p. 791-801, 2014. |
DOI: |
10.1007/s11295-013-0664-2 |
Idioma: |
Inglês |
Conteúdo: |
Quantitative trait loci (QTL) mapping is an important approach for the study of the genetic architecture of quantitative traits. For perennial species, inbred lines cannot be obtained due to inbreed depression and a long juvenile period. Instead, linkage mapping can be performed by using a full-sib progeny. This creates a complex scenario because both markers and QTL alleles can have different segregation patterns as well as different linkage phases between them. We present a two-step method for QTL mapping using full-sib progeny based on composite interval mapping (i.e., interval mapping with cofactors), considering an integrated genetic map with markers with different segregation patterns and conditional probabilities obtained by a multipoint approach. The model is based on three orthogonal contrasts to estimate the additive effect (one in each parent) and dominance effect. These estimatives are obtained using the EM algorithm. In the first step, the genome is scanned to detect QTL. After, segregation pattern and linkage phases between QTL and markers are estimated. A simulated example is presented to validate the methodology. In general, the new model is more effective than existing approaches, because it can reveal QTL present in a full-sib progeny that segregates in any pattern present and can also identify dominance effects. Also, the inclusion of cofactors provided more statistical power for QTL mapping. |
Palavras-Chave: |
Arquitetura genética. |
Thesagro: |
Genética; Progênie. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02039naa a2200217 a 4500 001 1994580 005 2017-05-23 008 2014 bl uuuu u00u1 u #d 024 7 $a10.1007/s11295-013-0664-2$2DOI 100 1 $aGAZAFFI, R. 245 $aA model for quantitative trait loci mapping.$h[electronic resource] 260 $c2014 520 $aQuantitative trait loci (QTL) mapping is an important approach for the study of the genetic architecture of quantitative traits. For perennial species, inbred lines cannot be obtained due to inbreed depression and a long juvenile period. Instead, linkage mapping can be performed by using a full-sib progeny. This creates a complex scenario because both markers and QTL alleles can have different segregation patterns as well as different linkage phases between them. We present a two-step method for QTL mapping using full-sib progeny based on composite interval mapping (i.e., interval mapping with cofactors), considering an integrated genetic map with markers with different segregation patterns and conditional probabilities obtained by a multipoint approach. The model is based on three orthogonal contrasts to estimate the additive effect (one in each parent) and dominance effect. These estimatives are obtained using the EM algorithm. In the first step, the genome is scanned to detect QTL. After, segregation pattern and linkage phases between QTL and markers are estimated. A simulated example is presented to validate the methodology. In general, the new model is more effective than existing approaches, because it can reveal QTL present in a full-sib progeny that segregates in any pattern present and can also identify dominance effects. Also, the inclusion of cofactors provided more statistical power for QTL mapping. 650 $aGenética 650 $aProgênie 653 $aArquitetura genética 700 1 $aMARGARIDO, G. R. A. 700 1 $aPASTINA, M. M. 700 1 $aMOLLINARI, M. 700 1 $aGARCIA, A. A. F. 773 $tTree Genetics & Genomes$gv. 10, p. 791-801, 2014.
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